Study on molecular regulation mechanism of VEGF in HL-60 cells after induced differentiation
10.3760/cma.j.issn.1673-422X.2012.011.023
- VernacularTitle:HL-60细胞诱导分化后血管内皮生长因子表达变化分子机制的探讨
- Author:
Yanmei LI
;
Guanhua SONG
;
Peie WEN
;
Xia REN
;
Kehong BI
;
Guosheng JIANG
- Publication Type:Journal Article
- Keywords:
Tretinoin;
Vascular endothelial growth factors;
HL-60 cells
- From:
Journal of International Oncology
2012;(11):873-877
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the molecular regulation mechanism of VEGF in the model of ATRA induced differentiation in HL-60 cells,and to provide new targets for leukemia anti-angiogenic therapy.Methods The morphology was observed by Wright-Gimesa staining; HL-60 cells differentiation was detected by NBT reduction experiment.VEGF,STAT3,c-myc mRNAs were measured by reverse transcription-PCR;VEGF,STAT3 and c-myc proteins were determined by Western blot.Results The proliferation of HL-60 cells was inhibited obviously by ATRA(1 μmol/L) with the induction of differentiation,NBT positive rate was 82.59％ (t =-24.157,P ＜ 0.01) ; VEGF mRNA (t =7.339,P ＜ 0.05),STAT3 mRNA (t =3.667,P ＜0.05) and c-myc mRNA (t =6.858,P ＜ 0.05) were all down-regulated.VEGF protein (t =3.386,P ＜0.05),STAT3 protein(t =4.074,P ＜ 0.05) and c-myc protein (t =3.333,P ＜ 0.05) were all down-regulated.Conclusion VEGF expression level is reduced with the procession of differentiation of HL-60 cells,which may be largely correlated with the down regulation of STAT3 and c-myc.
