Th1 Cytokines induced by ESAT-6 and CFP-10 in PBMCs from patients with refractory pulmonary tuberculosis were impaired
10.3760/cma.j.issn.1008-1372.2012.10.002
- VernacularTitle:难治性肺结核患者PBMCs结核分枝杆菌特异性Th1细胞因子反应低下
- Author:
Xinjing WANG
;
Zhihong CAO
;
Jinxin LIU
;
Bingfen YANG
;
Xiaoxing CHENG
- Publication Type:Journal Article
- Keywords:
Th1 cells;
Cytokines/blood;
Tuberculosis,pulmonary/immunology;
Mycobacterium tuberculosis
- From:
Journal of Chinese Physician
2012;(10):1300-1302
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the feature of Th1 cytokines induced by Mtb-specific antigen in patients with refractory pulmonary tuberculosis.Methods 28 patients with refractory pulmonary tuberculosis,67 patients with first-treated pulmonary tuberculosis,and 25 healthy controls with positive T.spot (LTBI group) were enrolled.IFN-γ,IL-2 and TNF-α in supernatants from PBMCs stimulated with ESAT-6 and CFP-10 were analyzed with Bender Flowcytomix on flow cytometry.Results The levels of the three cytokines were utmost high in patients with first-treated pulmonary tuberculosis.The lowest level of IFN-γ and IL-2 were induced in patients with refractory pulmonary tuberculosis,and were significantly lower than the LTBI group(Mann-Whitney U ＝ 105.5,162.5,P ＜ 0.01).Conclusions The immunotherapy with IFN-γ and IL-2 may play a role in treatment for refractory pulmonary tuberculosis but not for most of first-treated pulmonary tuberculosis.
