Effect of siRNA Interference of HRG-1 on bladder cancer T24 cells in vitro
10.3760/cma.j.issn.1008-1372.2012.10.015
- VernacularTitle:siRNA沉默人类血红素敏感基因1对膀胱癌T24细胞的影响
- Author:
Lei TANG
;
Ruixiao LI
;
Chuigong YU
;
Jianlin YUAN
;
Guojun WU
- Publication Type:Journal Article
- Keywords:
RNA,small interfering;
Urinary bladder neoplasms/pathology;
Gene expression regulation,neoplastic;
Gene silencing
- From:
Journal of Chinese Physician
2012;(10):1346-1350
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expression change of HRG-1 gene between urinary bladder carcinoma and normal tissues,and to investigate the effect of HRG-1-siRNA on the proliferation and apoptosis of human bladder carcinoma cells.Methods Immunohistochemisty was used to detect the expression of HRG-1 in 85 cases of bladder carcinomas and 20 normal bladder tissues.The siRNA of HRG-1 was designed,synthesized,and transfected into bladder cancer cell line T24.Results The HRG-1 gene expression had significant differences between bladder carcinoma and normal bladder tissues (P ＜ 0.05).The positive expression of HRG-1 gene had significant differences between the pathological grades and clinical stages of bladder carcinomas (P ＜0.05).After treated with siRNA,the expression levels of HRG-1 protein and mRNA in T24 cells decreased obviously (P ＜ 0.05).The apoptosis rate of T24 cells transfected with HRG-1-siRNA was significantly different from control-siRNA group and blank group (P ＜ 0.01).Conclusions The high expression of HRG-1 gene may play an important role in bladder carcinoma,and siRNA targeting HRG-1 can suppress HRG-1 protein expression markedly and enhance apoptosis of T24 cells.
