The clinical significance of the detection of hepatitis B virus large surface protein for the diagnosis and treatment
10.3760/cma.j.issn.1008-6706.2012.24.001
- VernacularTitle:乙型肝炎病毒外膜大蛋白检测的临床意义
- Author:
Xinmei JI
;
Haizhu LI
;
Hui DU
;
Siwu QIU
;
Boliang ZHAO
- Publication Type:Journal Article
- Keywords:
Hepatitis B large surface protein;
Hepatitis B virus;
Virus replication
- From:
Chinese Journal of Primary Medicine and Pharmacy
2012;(24):3681-3682
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the correlation between hepatitis B virus large surface protein(HBV-LP)and hepatitis B virus deoxyribonucleic acid(HBV-DNA),analyze the clinical significance of detecting the two index dynamically for diagnosis and treatment of hepatitis B.Methods Enzyme linked immunosorbent assay(ELISA)and fluorescent quantitation polymerase chain reaction(FQ-PCR)were used to detect the levels of HBV-LP and HBV-DNA in serum specimen of 230 hepatitis B patients.Results There was a positive correlation between the content of HBV-LP and copy numbers of HBV-DNA in serum of hepatitis B positive cases(r=0.84,P＜0.01).The positive rate was 84.78％ in HBV-LP and 84.35％ in HBV-DNA of 230 HBV positive cases.As a result there was no significant difference(P＞0.05)in this study.Therefore,the positive rate was 63.63 ％(35/55)in HBV-LP and 58.18％(32/55)in HBV-DNA of 55 HBV negative cases.There was also no significant difference(P＞0.05)in this study.However,the positive rate of HBV-LP(82.47％)was higher than HBE(52.06％)in the 194 HBV-DNA positive cases.There was significantly different(P＜0.01).Conclusion HBV-LP and HBV-DNA are the significant index of the degree of hepatitis B virus replication in hepatitis B positive cases,especially in determining the virus replication and prognosis of treatment in HBe Ag negative,providing the reliable laboratory data for the clinical diagnosis and treatment.
