Analysis of the expression in platelets membrane glycoprotein of the MYH9-RD family by flowcytometry
10.3760/cma.j.issn.1009-9158.2012.12.023
- VernacularTitle:采用流式细胞术分析MYH9-RD家系血小板膜糖蛋白的表达
- Author:
Shufang ZHANG
;
Shunlan WANG
;
Yingai ZHANG
;
Jingchuan XIAO
;
Denggao HUANG
- Publication Type:Journal Article
- Keywords:
Thrombocytopenia;
Platelet membrane glycoproteins;
Myosin heavy chains;
Molecular motor proteins;
Flow cytometry
- From:
Chinese Journal of Laboratory Medicine
2012;(12):1159-1162
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the pathogenic mechanism by detecting the expression of membrane glycoprotein in the platelets of nonmuscle myosin heavy chain 9 related disease (MYH9-RD)patients.Methods Periperal bloods were obtained from 11 MYH9-RD patients and 7 normal family members.Flow cytometry was used for detecting the expression of the platelet membrane glycoprotein including GP Ⅱ b/Ⅲa(CD41/61),GP Ⅰ a(CD49b),GP Ⅰ b/Ⅸ/Ⅴ (CD42a) GP Ⅰ b(CD42b) and GPⅣCD36).Results The expression fluorescence intensity of platelet membrane glycoprotein GP Ⅱ b/Ⅲ a CD41/61),GPⅠa(CD49b),GP Ⅰ b/Ⅸ/Ⅴ (CD42a) GP Ⅰ b(CD42b) and GPⅣ (CD36) are 653.7 ±192.7,420.0 ± 151.3,667.7 ± 371.3 and 236.4 ± 64.2 respectively,which are significantly higher than those in normal controls (406.7 ± 126.1,181.2 ± 29.3,271.4 ± 91.6 and 136.1 ± 23.5 ; P ＜ 0.01) ; The expression of GP Ⅰ a(CD49b) was lower in patients with MYH9-RD (139.1 ± 54.9) than that in normal controls (192.2 ± 143.4),but there was no significant difference (P ＞ 0.05).Conclusion In our study,the diverse clinical manifestations in patients with MYH9-RD is probably associated with the expression level of platelet membrane glycoprotein
