Interleukin 17 decrease the polarization of macrophage and attenuate the pathology of obliterative bronchiolitis after trachea transplant modulated
10.3760/cma.j.issn.1001-4497.2012.12.008
- VernacularTitle:白细胞介素-17对巨噬细胞分化的影响及在闭塞性细支气管炎中的作用
- Author:
Jiangang WANG
;
Xiaohui ZHOU
;
Yinkai NI
;
Qian SHI
;
Hao CAO
;
Huimin FAN
- Publication Type:Journal Article
- Keywords:
Transplantation;
Macrophages;
Bronchiolitis obliterans;
Models,animal;
Interleukin 17
- From:
Chinese Journal of Thoracic and Cardiovascular Surgery
2012;(12):732-734
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of interleukin 17 in the differentiation of macrophages from monocyte in vivo and its applicability in Obliterative bronchiolitis (OB).Methods Pathogen-free male C57BL/6 (n =20),IL-17-defcient C57BL/6 (n =10) and BALB/c (n =10) weighing 20-25 g.Weight-matched mice were assigned to three experimental groups.Experimental group A:BALB/c (n =5) →C57 BL/6 (n =10).Experimental group B:BALB/c (n =5) →IL-17-defcient C57BL/6(n =10).Normal control group:C57BL/6(n =10).The CD80 expression in macrophages in spleens were analysed by flowcytometry 7 days after operation.Native and transplanted lungs were harvested after 14 days and 28 days,stained with HE,and examined under light microscopy.Results Compared to experimental group A,CD80 expression of macrophages reveales a significant decrease in spleen of experimental group B.Furthermore,airway obliteration and destruction of the epithelium in experimental group A were significantly better compared with that of experimental group B on day 14 and 28 after transplantation.Conclusion IL-17 deficiency decreased the polarization of monocytes to macrophages and attenuate the pathology of obliteral bronchiolitis in murine model after trachea transplantaion.
