Effect of luxS mutation on the oxidative stress of Streptococcus mutans

VernacularTitle:luxS基因缺失对变异链球菌氧化应激的影响及机制初探
Author: Danni YU ; Ya ZHANG ; Wei ZHAO ; Na LI ; Yuzhi HAN
Publication Type:Journal Article
Keywords: Streptococcus mutans; luxS gene; Oxidative stress; Fenton reaction
From:Chinese Journal of Microbiology and Immunology 2012;(12):1057-1060
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of luxS inactivation on the oxidative stress of Streptococcus mutans and perform preliminary analysis of potential mechanism.Methods Strains were grown to mid-logarithmic phase and divided into three groups,one was used as control and inoculated into normal TPY medium,and the other two groups were experimental groups,and there were separately inoculated into TPY containing 58.8 mmol/L hydrogen peroxideor TPY containing 58.8 mmol/L hydrogen peroxide and 0.1 mmol/L 2,2'-dipyridyl.The survival rate of strain was calculated at 0.5,1,and 2 h.All the data were statistically analyzed.Results Compared with the control group,the survival rate of luxS mutation was always higher than standard strain at all pre-determined time inexperimental groups (P＜0.05),and compared with experimental group without iron chelator,the survival rate of strains was not raised with the added of iron chelator (P＞0.05).Conclusion luxS gene is involved in oxidative stress tolerance of Streptococcus mutans,and the oxidative stress tolerance is not achieved by avoiding the toxic effects of the Fenton reaction