Preliminary study of the rabies virus glycoprotein eukaryotic expression plasmid and immunogenicity
10.3760/cma.j.issn.0254-5101.2012.12.005
- VernacularTitle:狂犬病病毒糖蛋白真核表达质粒的构建及免疫原性的初步研究
- Author:
Yunpeng WANG
;
Shouchun CAO
;
Jia LI
;
Jinghua LIU
;
Leitai SHI
;
Yuhua LI
;
Guanmu DONG
- Publication Type:Journal Article
- Keywords:
Rabies virus;
Glycoprotein;
Immunogenicity
- From:
Chinese Journal of Microbiology and Immunology
2012;(12):1017-1019
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct the eukaryotic expression plasmid of the rabies virus glycoprotein gene DNA,and detect the immunogenicity.Methods Using RT-PCR amplified the glycoprotein gene of rabies virus CTN strain,sequenced and cloned into pcDNA5.0 (+) vector to construct the recombinant plasmid pcDNA5.0-G plasmid.Detect glycoprotein transient expression with transfecting the plasmid into 293T cells.Intramuscular immunization of BALB/c mice by the recombinant plasmid on day 0 and 7,then challenge by rabies virus CVS strain observed the mice survived.Results The results of the transient expression of glycoprotein abundantly expressed.The survival ratio of mice with CVS challenge after routine intramuscular injection of pcDNA5.0-G plasmid is 73.3％,and 6.7％ for the control group.Conclusion Rabies virus glycoprotein eukaryotic expression plasmid pcDNA5.0-G was successfully constructed,and has been good immunogenicity.It's to be the foundation for candidate DNA vaccine research and development.
