Identification of a novel human leukocyte antigen allele, HLA-A * 31 ∶ 22
10.3760/cma.j.issn.0254-5101.2012.12.002
- VernacularTitle:人类白细胞抗原新等位基因HLA-A*31∶22的序列分析及确认
- Author:
Xin QI
;
Guiji LI
;
Xu ZHANG
;
Kunlian ZHANG
;
Xiaofeng LI
- Publication Type:Journal Article
- Keywords:
HLA-A * 31 ∶ 22;
Novel allele;
Sequencing-based typing
- From:
Chinese Journal of Microbiology and Immunology
2012;(12):1011-1014
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify and confirm a novel HLA allele.Methods A new human leukocyte antigen A allele was found during routine HLA genotyping by polymerase chain reaction-sequence specific oligonucleotide probes(PCR-SSOP) and sequencing-based typing (SBT).HLA-A locus was amplified from exon 1 through exon 8,and the nucleotide sequence of exon 2 to exon 4 for HLA-A were sequenced in both directions.Results The novel HLA-A * 31 allele is identical to A * 31 ∶ 01 ∶ 02 with an exception of one base substitution at position 245 of exon 2 where an ' A' change to ' C' resulting in codon 82 changed from GAG to GCG.Conclusion A novel HLA allele,A * 31 ∶ 22,was identified,and was named officially by the WHO Nomenclature Committee for factors of the HLA system.
