Heat-killed Staphylococcus aureus induces a common early response in human monocytes in the presence of high concentration glucose
10.3760/cma.j.issn.0254-5101.2012.12.001
- VernacularTitle:高糖预处理影响单核细胞对热灭活金黄色葡萄球菌刺激的反应
- Author:
Ying CHEN
;
Yan ZHANG
;
Xin SONG
;
Pei SUN
;
Bai CHANG
;
Haidong LI
;
Dong MENG
;
Qiaofen LI
- Publication Type:Journal Article
- Keywords:
High concentrations of glucose;
Monocytes;
Heat-killed Staphylococcus aureus (HKSA);
Apoptosis;
iNOS;
IL-1β
- From:Chinese Journal of Microbiology and Immunology
2012;(12):1005-1010
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of heat-killed Staphylococcus aureus (HKSA) on the apoptosis and expression of iNOS and IL-1β in THP-1 monocytes in the presence of high concentration of glucose.Methods THP-1 cells were cultured in medium containing 25.0 mmol/L(HG) or 5.5 mmol/L (LG,control) D-glucose for 12 h-8 d.The THP-1 cells cultured for 6 d were extracted on the 0-48 h with or without HKSA,then apoptosis and expression of iNOS and IL-1β were examined.Apoptosis was analyzed by flow cytometry and expressions of IL-1β and iNOS were quantitated by real-time PCR.Results The expression of iNOS and IL-1β in THP-1 monocytes was increased significantly in the presence of high concentration of glucose for 12-48 h(P＜0.05),reaching the highest level at 24 h and returned to baseline after 4 d.The expression was significantly lower than that of control after 4-6 d.Apoptosis rate was also increased significantly after 48 h to 4 days.HKSA infection enhanced apoptosis,but inhibited the expression of iNOS and IL-1 β in the presence of high concentration of glucose.The expression of iNOS and IL-1β increased significantly at 6 h(P＜0.01),reaching the highest level at 12 h,but the levels were significantly lower than those in control groups (P＜0.05).Conclusion These data suggest that high concentration of glucose can interfere with the anti-bacterial function of monocytes by reducing their expression of iNOS and IL-1β and enhancing their apoptosis.
