IL-22 is involved in atherosclerosis lesions by regulated impaired proliferation ability of oxidized low density lipoprotein treated CRL-1730
10.3760/cma.j.issn.0254-5101.2012.11.016
- VernacularTitle:IL-22在氧化型低密度脂蛋白损伤内皮细胞中的调节作用
- Author:
Yi SUN
;
Zhijun HAN
;
Yuanlan HUANG
;
Mingli GU
;
Yan CHEN
;
Zhide HU
;
Anmei DENG
;
Renqian ZHONG
- Publication Type:Journal Article
- Keywords:
Atherosclerosis;
Interleukin-22;
Human umbilical vein endothelial cell;
Oxidized low density lipoprotein;
Proliferation;
Basic fibroblast growth factor
- From:Chinese Journal of Microbiology and Immunology
2012;(11):995-999
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the association between IL-22 and the pathogenesis of coronary artery atherosclerosis(AS).Methods The relative expression of IL-22 mRNA in PBMC from 30 AS patients and 8 patients without any signs of coronary artery stenosis was detected by RT-PCR.Serum IL-22 levels of 22 patients without any signs of coronary artery stenosis and 79 AS patients were detected by ELISA.CRL-1730 cells(human umbilical vein endothelial cells) were stimulated with oxidized low density lipoprotein (ox-LDL) at different dosage for 24 h,and the expression of IL-22R1 was detected by flowcytometry.The proliferation ability of CRL-1730 cells treated with IL-22(20 ng/ml) and/or ox-LDL(100 μg/ml)was measured by MTS assay,and the expression of basic fibroblast growth factor(bFGF) was detected by RTPCR and ELISA.Results Decreased IL-22 expression in PBMC and serum was observed as worsen of AS.The expression of IL-22R1 in ox-LDL treated CRL-1730 cells was increased in dose dependent manner.OxLDL decreased proliferation ability,as well as bFGF expression and releasing,of CRL-1730 cells.This effect of ox-LDL was partially rescued by IL-22.Conclusion IL-22 may have anti-atherosclerosis effect.This effect may be mediated by regulating bFGF expression and endothelial cells proliferation ability in the presence of IL-22.
