Detection of beta-lactamase, porin-coding genes and linkage of KPC-ISKpn6 of the carbapenems-resistant Klebsiella pneumoniae
10.3760/cma.j.issn.0254-5101.2012.11.008
- VernacularTitle:耐碳青霉烯类药物肺炎克雷伯菌β-内酰胺酶与膜孔蛋白编码基因以及KPC-ISKpn6连锁检测
- Author:
Jin CUI
;
Ganzhu FENG
;
Liangmei WANG
;
Shuidi ZHAO
;
Yang ZHANG
;
Tianming GAO
- Publication Type:Journal Article
- Keywords:
Klebsiella pneumoniae;
β-1actamase;
Carbapenems-resistant;
Porin;
Insertion sequence
- From:
Chinese Journal of Microbiology and Immunology
2012;(11):953-958
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the resistance-mechanism of the carbapenems-resistant Klebsiella pneumoniae isolated from clinical.Methods The clinical isolates of carbapenems-resistant Klebsiella pneumoniae from top three comprehensive hospitals of Nanjing area were examined by 40 beta-lactamase,porin-coding genes and linkage of KPC-ISKpn6 using PCR method,the PCR positive results were picked out for sequencing and sequencing BLAST search for comparison analysis.Results Twenty-four strains of carbapenems-resistant Klebsiella pneumoniae were detected,the positive rate of A beta-lactamase TEM-1 and SHV was 100％ (24/24),KPC-2 and LAP-2 was 95.8％ (23/24),45.8％ (11/24) respectively,and C beta-lactamase DHA was 4.2％ (1/24).Meanwhile,the positive detection rates of KPC-ISKpn6 linkage was 95.8％ (23/24),and the mutation rate of porin-coding genes ompK35 and ompK36 were up to 95.8％ (23/24) and 100％ (24/24).Conclusion High incidence of beta-lactamase TEM-1,SHV,KPC-2 and LAP-2 was found in the group of Klebsiella pneumoniae isolates,and carbapenems-resistant of which was primarily due to the high carrying rate of KPC-2 and the high mutation rate of porin-coding genes ompK35 and ompK36.The Insertion sequence ISKpn6 may be involved in the KPC-2 gene mediated-expression.
