The study of the relationship between influenza virus infection and the dysfunction of vascular smooth muscle cells
10.3760/cma.j.issn.0254-5101.2012.10.001
- VernacularTitle:流感病毒感染致大鼠血管平滑肌细胞功能变化的研究
- Author:
Xiuru GUAN
;
Xinyuan JIA
;
Xiaochen YU
;
Na ZHANG
;
Yuling JIANG
;
Xiaomin XIN
- Publication Type:Journal Article
- Keywords:
Atherosclerosis;
Influenza virus;
Smooth muscle cells;
Cell proliferation;
Cell apoptosis
- From:Chinese Journal of Microbiology and Immunology
2012;(10):841-844
- CountryChina
- Language:Chinese
-
Abstract:
Objective To research the influenza virus infection on rat vascular smooth cells number,proliferation,apoptosis,the amount of IL-6,sFas,platelet-derived growth factor(PDGF) and the mechanism of atherosclerosis.Methods Flow cytometry,enzyme-linked immunosorbent assay and cell count experiments were used to detect these indicators at 0 h,6 h,12 h,24 h,48 h.Results After influenza virus infected at 0 h,proliferation,apoptosis condition were 10.39％,0.44％,respectively; at 6 h,proliferation,apoptosis respectively increased to 12.68％,0.73％ ; proliferation reached the peak at 12 h (18.01％),instead apoptosis decreased to 0.14％ ; at 24 h,proliferation decreased to 12.89％ and apoptosis markedly increased to 1.09％ ; at 48 h,proliferation further reduced to 7.07％ and apoptosis reached the peak(4.61％).The number of cells and the cytokine secretion were statistically significant to control group (P＜0.05).Conclusion Influenza virus infection might lead to change of cell proliferation and apoptosis and involve the atherosclerosis form and development,and cytokines played an important role in them.
