Effect of hepatitis B virus X gene on transdifferentiation of human proximal tubular epithelial cells
10.3760/cma.j.issn.1001-7097.2012.12.010
- VernacularTitle:乙型肝炎病毒X基因对肾小管上皮细胞间质转分化的影响
- Author:
Yi ZHOU
;
Xuan WANG
;
Weijie YUAN
;
Nan ZHU
- Publication Type:Journal Article
- Keywords:
Transfection;
Inflammation;
Hepatitis B virus;
Renal tubular epithelial cells;
Transdifferentiation
- From:
Chinese Journal of Nephrology
2012;(12):956-960
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of hepatitis B virus X (HBX) gene on cell morphology and transdifferentiation of human proximal tubular epithelial cells.Methods The eukaryotic vector pcDNA3.1-myc-HBX containing HBX gene was transiently transfected into HK-2 cells by lipofectamine mediation.The expression of HBX was confirmed by Q-PCR and Western blotting.Untransfected HK-2 cells and those transfected with empty vector were used as control.The morphology of HK-2 cells was observed by microscopy,the expressions of differentiation marker proteins α-SMA and E-cadherin were detected by Western blotting and Q-PCR,and the contents of IL-1,IL-6 and TNF-α in the supernatant were detected by ELISA assay.Results HBX was successfully expressed in HK-2 cells after transfection.After transfection of HBX gene,the shape of HK-2 cells became irregular,HK-2 cells significantly expressed E-cadherin and α-SMA,and had high levels of IL-1,IL-6 and TNF-α in the cell supernatant (P＜0.01).Conclusion Overexpression of HBX gene in renal tubular epithelial cells may damage cell morphology and promote the occurrence of epithelial-mesenchymal transdifferentiation,which may be related to the inflammatory microenvironment.
