Fluvastatin attenuates the down-regulation of β1 integrin expression in PAN-treated podocytes by inhibiting ROS
10.3760/cma.j.issn.1001-7097.2012.11.001
- VernacularTitle:氟伐他汀通过抑制活性氧改善氨基核苷嘌呤霉素作用下足细胞β1整合素的表达
- Author:
Jia LIU
;
Changying XING
;
Yuping CHAI
;
Yaguang XU
;
Xiufen ZHAO
;
Jun QIAN
;
Bin SUN
;
Xiaoyun WANG
- Publication Type:Journal Article
- Keywords:
Podocyte;
Antigen,29;
Puromycin,aminonucleoside;
Fluvastatin;
Reactive oxygen species
- From:
Chinese Journal of Nephrology
2012;(11):829-834
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of fluvastatin (FLV) on the expression of β1 integrin in puromycin aminonucleoside (PAN)-treated podocytes and its mechanism.Methods Cultured human podocytes were divided into PAN,different concentrations of fluvastatin (1 × 10-8 to 1 × 10-5 mol/L),SOD,H2O21 groups respectively.Expressions of β1 integrin and reactive oxygen species (ROS) in podocytes were detected by Western blotting and DCFHDA (2' 7'-Dichlorofluoresecein 3' 6'-diacetate) respectively.The viability of podocyte was determined by MTT colorimetry.Results PAN and H2O2 significantly decreased the expression of β1 integrin and increased the synthesis of ROS in podocytes (P＜0.05respectively).Lower concentration fluvastatin or SOD treatment up-regulated β1 integrin and downregulated ROS of podocytes induced by PAN (P＜0.05 respectively).MTT revealed that lower podocyte viability was found in higher concentration fluvastatin,PAN and H2O2 groups.Lower concentration fluvastatin and SOD could protect podocytes against PAN.Conclusion Fluvastatin attenuates the injury of podocyte induced by PAN and increases the expression of β1 integrin,whose mechanism may be associated with the inhibition of the ROS activity.
