Protection of NGAL on renal tubular epithelial cells in rat renal ischemia reperfusion injury
10.3760/cma.j.issn.1001-7097.2012.10.012
- VernacularTitle:中性粒细胞明胶酶相关脂质运载蛋白对大鼠肾脏缺血再灌注损伤肾小管上皮细胞的保护作用
- Author:
Xiujuan ZANG
;
Li GONG
;
Haijuan HONG
;
Yan JIANG
;
Feng ZHENG
;
Mei LIU
;
Qing YU
- Publication Type:Journal Article
- Keywords:
Reperfusion injury;
Apoptosis;
Renal tubular epithelial cells;
Apoptosis-regulated protein;
Neutrophil gelatinase associated lipocalin
- From:
Chinese Journal of Nephrology
2012;(10):804-807
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of neutrophil gelatinase associated lipocalin(NGAL) on renal tubular epithelial cells apoptosis and apoptosis-regulated protein fas,bcl-2 in rat renal ischemia reperfusion injury (IRI).Methods Renal IRI models of rats were established.Rats were randomly divided into 3 groups,including control group,IRI model group and NGAL group.The pathological change of kidney tissue was investigated by hemotoxylin-eosin staining.Renal tubular epithelial cells apoptosis was detected by TUNEL method.Expression of fas and bcl-2 was measured by real-time PCR and Western blotting.Results Compared with IRI model group,NGAL group showed a decreased number of renal tubular epithelial cells apoptosis [(8.6±3.4)/HP vs (20.8±3.7)/HP,P＜0.05],down-regulated fas mRNA (2.34±0.51 vs 6.84±2.34,P＜ 0.05),fas protein (0.65±0.05 vs 0.95±0.08,P＜0.05) and up-regulated bcl-2 protein (0.33±0.05 vs 0.24±0.03,P＜0.05),but the bcl-2 mRNA had no significant change.Conclusion NGAL can protect renal tubular epithelial cells in renal IRI,which may be associated with decreasing cell apoptosis and adjusting protein expression by apoptosis-regulated cytokines.
