Effect of Perfadex and Wisconsin Unviersity solution on the function of pulmonary artery endothelium
10.3760/cma.j.issn.0254-1785.2013.02.012
- VernacularTitle:Perfadex液与UW液对猪肺动脉内皮细胞功能的影响
- Author:
Zhiqiang FENG
;
Shikang LI
;
Yingxue HU
;
Qiangxin HUANG
;
Qiyun ZHOU
;
Lishu HE
- Publication Type:Journal Article
- Keywords:
Pulmonary artery;
Endothelium-derived hyperpolarizing factor;
Perfadex solution;
Wisconsin Unviersity solution
- From:
Chinese Journal of Organ Transplantation
2013;(2):114-116
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of Perfadex and Wisconsin Unviersity solution on the function of pulmonary artery endothelium.Methods Small lobe pulmonary arteries were dissected from nine porcine lungs.The artery from each lung was cut into six rings in 2mm.Two of them were randomly incubated in Krebs,Perfadex or Wisconsin Unviersity solution (UW solution) at 4℃ for 4 hours.Endothelium-derived hyperpolarizing factor (EDHF)-mediated relaxation (percentage of-7.5 logM U46619 precontraction) induced by bradykinin or calcium ionophone A23187 in the present of indomethacin,L-NNA and oxyhemoglobin were measured at 37 ℃ in the organ chambers.Results Vasoconstriction induced by U46619 is no significant difference among three groups (P＞ 0.05).Compared with Krebs group,the relaxation induced by bradykinin or A23187 was significantly decreased in Perfadex group (P＜0.01),while there is no significant difference in UW group (P＞0.05).Conclusion Endothelium-derived hyperpolarizing factor (EDHF) plays an important role in endothelium-mediated relaxation of porcine pulmonary artery.EDHF-mediated relaxation is impaired when the lung preserved with Wisconsin Unviersity solution,wheras its function is not affected by Perfadex solution.
