Immunogenicity, immune mechanisms and immune modulatory effects of mesenchymal stem cells derived from bone marrow of patients with multiple myeloma
10.3760/cma.j.issn.0254-1785.2013.02.011
- VernacularTitle:多发性骨髓瘤患者间充质干细胞的免疫原性及其对免疫功能的调控作用
- Author:
Liping BAI
;
Ying ZHANG
;
Jinhuan WANG
;
Zhenling WANG
;
Zhigang ZHAO
- Publication Type:Journal Article
- Keywords:
Multiple myeloma;
Mesenchymal stem cells;
Immunregulation;
Cytokines
- From:
Chinese Journal of Organ Transplantation
2013;(2):110-113
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the immunogenicity,immune modulatory effects and mechanisms of mesenthymal stem ceils (MSCs) derived from the bone marrow of patients with multiple myeloma (MM).Methods The mononuclear cells from the bone marrow of MM patients were obtained and cultured.Immunophenotypes were investigated by using FACS.The levels of cytokines were determined by using enzyme linked immunosorbant assay (ELISA).The endocytosis of monocyte-derived dendritic cells (DCs) was investigated by using FACS.Moreover,the immunoregulatory ability of DCs on proliferation of T lymphocytes was detected by using mixed lymphocyte culture assay.Results MM-derived MSCs had no expression of HLA-DR and costirnulatory molecules (CD40,CD80,CD83 and CD86).MM-derived MSCs could significantly suppress proliferation of T lymphocytes in a dose-dependent manner,which could be reversed by antiTGF-β1 and anti-HGF antibodies.MM-derived MSCs inhibit the endocytosis of DCs.MM-derived MSCs could inhibit the secretion of IL-12 and significantly inhibit the function of DCs on proliferation of T lymphocytes.Conclusion MM-derived MSCs harbored low immunogenicity and immunoregulatory effect in vitro,and this effect was achieved through cytokines.
