Expression of protease activated receptors-2 in intestinal mucosa of acute necrotizing pancre-atitis in rats
10.3760/cma.j.issn.0254-1432.2012.09.008
- VernacularTitle:蛋白酶激活受体-2在急性坏死性胰腺炎大鼠肠黏膜组织中的表达
- Author:
Liang XIA
;
Jiang CHEN
;
Pi LIU
;
Tao SU
;
Ying HU
;
Nonghua Lü
- Publication Type:Journal Article
- Keywords:
Pancreatitis,acute necrotizing;
Intestinal mucosa;
Receptors,proteinaseactivated;
Disease models,animal
- From:
Chinese Journal of Digestion
2012;32(9):598-601
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the correlation between the expression of protease activated receptors-2 (PAR-2) and intestinal mucosal barrier injury of acute necrotizing pancreatitis (ANP) in rats.Methods The ANP rat model was created.The expression of PAR-2 in rat's intestinal mucosa of sham-operated group and ANP group at six,12 and 24 hours after model established was detected by immunohistochemistry (IHC),reverse transcription-polymerase chain reaction (RT-PCR) and Western blot.The difference between groups was analyzed by one way analysis of variance.Results The results of IHC indicated that PAR-2 expression in rat's intestinal mucosa of sham-operated group was weak.The number of PAR-2 expression positive cells and immunostaining intensity increased significantly after ANP model established.The IHC score was 4.88±0.33,5.87±0.32 and 11.17±0.27 at six,12 and 24 hours after model established respectively.Compared with those of shamoperated group (2.86 ± 0.31),the differences were statistically significant (F=747.08,P＜0.01).The expression of PAR-2 at mRNA and protein level in intestinal mucosa of sham-operated group was very low.As time extended after ANP model established,both expression increased gradually.The PAR-2 mRNA was 0.56±0.03,0.69±0.03,1.05±0.05,and the protein was 0.28±0.02,0.35±0.03,0.69±0.04 at six,12 and 24 hours after model established respectively.Compared with shamoperated group,the differences were statistically significant at each time point (F=785.69,1177.82,both P＜0.01).Conclusions PAR-2 is activated in the inflammatory progress of ANP,and may play an important role in the pathogenesis and development of intestinal mucosa barrier injury in ANP.
