Rapid detection of common bacterial infections of cerebrospinal fluid by genetics approach

VernacularTitle:基因学快速检测脑脊液常见细菌感染的方法
Author: Yixiang GUAN ; Jianhong SHEN ; Xingyun JU ; Demo WU ; Jinrong DING ; Yueping ZHONG ; Mingfei ZHANG ; Chunxiu ZHANG
Publication Type:Journal Article
Keywords: Central nervous system bacterial infections; Cerebrospinal fluid; Oligonucleotide array sequence analysis; Multiplex polymerase chain reaction; Drug resistance,bacterial
From: Chinese Journal of Neurology 2012;45(8):586-589
CountryChina
Language:Chinese
Abstract: ObjectiveTo assess gene chip application value in detecting pathogenic bacteria in intracranial infection cases.MethodsPrimers and probes aiming at the specific DNA sequences of 4 kinds of common pathogenic bacteria and 6 kinds of common drug resistance genes (DRGs) were designed and used to identify the bacteria and DRGs among 30 cerebrospinal fluid (CSF) specimens (12 positive,18negative in CSF culture) from patients with intracranial infection using multiplex polymerase chain reaction (mPCR) and gene chip.The results of gene detection were compared with those of CSF culture and drug sensitivity testing.ResultsBacteria were identified and DRGs were detected in 15 specimens; DRGs and 16S gene were detected in 8 specimens; neither bacterium nor DRG was detected in 7 specimens.ConclusionGene chip technique is characterized by its relative sensitivity and rapidity of detecting the pathogenic bacteria in CSF of intraeranial infection cases.