Effects of C-pseudonucleosides bearing thiazolidin-4-one as immunostimulants on differentiations of human lymphocytes
10.3760/cma.j.issn.0254-5101.2012.06.002
- VernacularTitle:含噻唑烷-4-酮的糖类衍生小分子免疫调节剂对人淋巴细胞分化的影响
- Author:
Ming MENG
;
Chunxiao LI
;
Yang HONG
;
Jinku ZHANG
;
Hongmei LIU
;
Dongzhi CHEN
;
Hua CHEN
;
Xiaoliu LI
- Publication Type:Journal Article
- Keywords:
Cytokines;
T cells;
B cells;
NK cells;
Immunostimulants
- From:
Chinese Journal of Microbiology and Immunology
2012;32(6):486-490
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of C-pseudonucleosides bearing thiazolidin-4-one as immunostimulants on differentiation and activation of human lymphocytes. Methods Peripheral blood mononuclear cells (PBMC) were isolated from healthy adults,and then incubated with immunostimulants (CH1a,CH2a,CH1b,CH2b and pidotimod).After 48 h,we collected the supernatants and then detected the concentrations of IL-2,IL-4 and IFN-γ using ELISA.After 72 h,the proliferation was detected using MTT method.PBMC incubated with immunostimulants (CH1a,CH2a,CH1b,CH2b and pidotimod),after 72 h,the cultural cells were collected and CD expressions of lymphocytes were analyzed by flow cytometry.Results All samples could stimulate proliferation of T cells.Immunostimulants CH1a,CH2a and pidotimod could elevate the expressions of CD3,CD4,CD19 and CD16CD56,and stimulate the secretions of IL-2 and IFN-γ. Immunostimulants CH1b and CH2b could elevate the expressions of CD3,CD4,CD19 and CD16CD56,and stimulate the secretions of IL-2 and IL-4. Conclusion Immunostimulants CH1a and CH2a could differentiate Th0 into Th1 and promote the proliferation of B cells as well as NK cells.However,immunostimulants CH1b and CH2b could differentiate Th0 into Th2 and promote the proliferation of B cells and NK cells.
