Molecular epidemiology of human Bocavirus infection in childhood diarrhea in Urumqi area

VernacularTitle:乌鲁木齐地区腹泻患儿中人博卡病毒感染分子流行病学调查
Author: Qifeng LI ; Bin ZHANG ; Naer JIA ; Liang YU ; Caixia LIU ; Zhaojun DUAN ; He SUN
Publication Type:Journal Article
Keywords: Human Bocavirus; Diarrhea; Children; Nested PCR
From: Chinese Journal of Microbiology and Immunology 2012;32(6):508-511
CountryChina
Language:Chinese
Abstract: Objective To analysis the molecular epidemiology characteristics of human Bocavirus1-4 ( HBoV 1-4) in children for diarrhea in Urumqi area.Methods Feces samples were collected from 315 in-patient and out-patient children with diarrhea at People's Hospital of Xinjiang Uygur Autonomous Region,Xinjiang Province,China,all through the year of 2011.Using nested PCR,which amplified NS1(518 bp) fragments.Human Bocavirus1-4 were screened. Results The overall frequency of HBoVs was 8.57％ (27/315),of which 2 were HBoV1,22 were HBoV2,and 3 were HBoV3.HBoV4 was not detected.Except XJ1378,the rest of 26 strains shared 98％-100％ nucleotide sequence identity with different reference strains,but 3 HBoV3 all shared 92％ nucleotide sequence identity with gorilla BGoV12009( HM145750.1 ).Phylogeny showed that NS1 fragments of HBoV3 were closer to that of HBoV1.HBoV infection was distributing throughout the year,there was no significant seasonal.There was no difference in gender,age and ethnic.Conclusion HBoV1-3 were detected throughout the year in Urumqi area,Xinjiang,HBoV2 was dominant.