The role of calcium in the rVvhA induced THP-1 cells apoptosis
10.3760/cma.j.issn.0254-5101.2012.06.007
- VernacularTitle:钙离子对重组创伤弧菌溶细胞素诱导THP-1细胞损伤的影响机制
- Author:
Yongming WANG
;
Qiu GUO
;
Bin SHI
;
Danli XIE
;
Yongliang LOU
- Publication Type:Journal Article
- Keywords:
Vibrio vulnificus;
Cytolysin;
Calcium;
THP-1
- From:
Chinese Journal of Microbiology and Immunology
2012;32(6):512-518
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the role of Vibrio vulnificus cytolysin(rVvhA) induced THP-1 apoptosis and calcium influx.Methods CCK-8 cell proliferation kit,Fluo3/AM staining and AnnexinV/PI staining were performed to identify the apoptosis and calcium influx induced by rVvhA in THP-1 cells.Results rVvhA could induce THP-1 apoptosis and up-regulate the cellular calcium concentration.BAPTAAM could enhance the calcium influx induced by rVvhA in THP-1.Conclusion rVvhA had cytotoxic to THP-1 cells by inducing apoptosis and triggering extracellular calcium influx.
