Effect of Schwann cell-derived neurotrophic factor on the biological characteristics of skeletal satellite cells in rats

VernacularTitle:许旺细胞源神经营养因子对大鼠肌卫星细胞体外活性的影响
Author: Jiang WU ; Min ZHU ; Chongtao ZHU ; Bin CHEN ; Xinmin XU ; Yongqin XU ; Yangjun OU ; Hua LIAO ; Xiaozhong QIU ; Jiakai ZHU ; Shuifa PANG
Publication Type:Journal Article
Keywords: Satellite cells; Schwann cell-derived neurotrophic factor; Proliferation; Differentiation
From: Chinese Journal of Microsurgery 2012;35(4):299-302
CountryChina
Language:Chinese
Abstract: Objective To study the effects of Schwann cell-derived neurotrophic factor(SDNF) on myoblast stem cells(called satellite cells,SCs) in vitro. Methods After setting up SCs culture system in vitro, SCs which treated with various SDNF concentrations culture medium were dynamically evaluated by cell morphology,MTT growth curve and fusion rate. Results The ability of SCs preceding their participation in muscle repair include proliferation and differentiation, 200 ng/ml SDNF stimulated cell proliferation more than the other medium,but 50 ng/ml,100 ng/ml,200 ng/ml,400 ng/ml SDNF made SCs differentiation significantly for their high myotube fusion rate. Conclusion SDNF can regulate the proliferation and differentiation of rat skeletal satellite cellsin vitro,but in differentiation significantly.SDNF might play a role in slowing down denervated muscle atrophy.