Effect of mixed-skin grafting with autologous microskin and allogenetic acellular dermal matrix microskin on fibronectin and laminin of wound surface in rats
10.3760/cma.j.issn.1671-0290.2012.04.015
- VernacularTitle:自体微粒皮与异体脱细胞微粒真皮混合移植对大鼠创面纤维连接蛋白和层粘连蛋白表达的影响
- Author:
Xuhui ZHANG
;
Yingbin XU
;
Yong HUANG
;
Suihang ZHONG
;
Peihong LIANG
- Publication Type:Journal Article
- Keywords:
Autologous microskin;
Allogenetic acellular dermal matrix microskin;
Mixed skin grafting;
Fibronectin (FN);
Laminin (LN)
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2012;18(4):290-293
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of mixed-skin grafting with autologous microskin and allogenetic acellular dermal matrix microskin on wound healing in rats,and to make a further study on the related mechanism.Methods Wistar rats were served as a allogenetic acellular dermal matrix donor rats,and SD rats as acceptors with mould of full thickness skin defects on their back.The ninety SD rats were divided into 5 groups with 18 rats in each group.Group 1 was transplanted with autologous microskin,and group 2 with allogenetic acellular dermal matrix microskin.Groups 3,4 and 5 were grafted with mixed-skin ratio between autologous microskin and allogenetic acellular dermal matrix microskin 1 ∶ 1,1 ∶ 0.5 and 1 ∶ 0.25,repectively.The rate of wound healing was measured,wound samples collected,hematoxylin and eosin stain carried out,fibronectin (FN) and laminin (LN)detected,and intergroup comparison made,respectively,2,3 and 4 weeks after skin grafting.Results The wound healing rates and FN and LN expression of mixed-skin grafting groups were higher than those of the group with autologous microskin grafting.The group of 1 ∶ 0.25 obviously increased (P＜0.05 or P＜0.01).Conclusions The wound healing rate with mixed-skin grafting is higher than that with autologous microskin grafting.The best effect is achieved when the skin ratio between autologous microskin and allogenetic acellular dermal matrix microskin is 1 ∶ 0.25.It is possibly due to the increase of FN and LN on wound skin surface.
