Studying the pathogenicity of TBEV to human neuroblastoma tumor cells
10.3760/cma.j.issn.0254-5101.2012.05.008
- VernacularTitle:TBEV对人神经细胞致病性的实验研究
- Author:
Jingjing WEI
;
Yuchang LI
;
Xiaoyan WU
;
Bingyin SI
;
Yu ZHANG
;
Jing LI
;
Yi HU
;
Qingyu ZHU
;
Yinhui YANG
;
Xiaoping KANG
- Publication Type:Journal Article
- Keywords:
Tick-borne encephalitis virus;
Neuroblastoma tumor cells;
Pathogenicity
- From:
Chinese Journal of Microbiology and Immunology
2012;32(5):415-418
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the infection and the replication of Tick-borne encephalitis virus(TBEV) in human neuroblastoma cells.Methods After being inffected with TBEV,the cell culture supernatant of human neuroblastoma cell line SK-N-SH was collected and assayed at different time points.Byusing real-time RT-PCR and plaque assay to measure the titer of virus in the supernatant,the replication andproliferation of TBEV in human neuroblastoma cell was identified.Meanwhile,the morphological change of SK-N-SH after TBEV infection was also visualized by observation under microscope and immunmquorescenceassay.Results Real-time RT-PCR and plaque assay both demonstrated that TBEV could replicate effectively in SK-N-SH cells,the peak titer could reach 2.92× 107 PFU/ml on 3 days post-inoculation.And significant morphological change occured on infected SK-N-SH cells after 2 days post inoculation.By immunofluorescence assay,the virus particles could be detected and visualized.Conclusion TBEV can replicate andproliferate effcctively and cause significant cell morphological changes in human neuroblastoma cell SK-N-SH,which demonstrated that SK-N-SH could be a suitable cell model for TBEV culture.
