Impact of B8R elimination on vaccinia virus induced immune responses

VernacularTitle:去除痘苗病毒显性表位B8R对外源抗原免疫原性的增强
Author: Xueyao LIU ; Di WU ; Jie XU ; Yang WANG
Publication Type:Journal Article
Keywords: Vaccinia virus; Immunodominance; Dominant epitope; Cellular immunity; Vaccine vector
From: Chinese Journal of Microbiology and Immunology 2012;32(5):425-429
CountryChina
Language:Chinese
Abstract: Objective To investigate the antigenicity of foreign antigen in recombinant vaccinia virus (VACV) after elimination of B8R,and provide help to improve the efficacy of VACV-based vaccines,and provide guidance for vaccine design.Methods Transfer vector pSC11-OVA was generated,and OVAgene was in,rted into VACV with B8R deletion.The biological characteristics of recombinant VACV was investigated in vitro,and the immune responses against OVA were tested in vivo.Results The plague phenotypes and growth of wcombinant VACV and its parental strains were essentially identical.Cellular immuneresponse against OVA was augmented in mice infected with B8R-deleted recombinant VACV when comparedwith those infected with B8R-intact recombinant VACV.Conclusion Deletion of B8R and insertion of OVAdoes not affect the biological characteristics of VACV.Immunogenicity of exogenous target antigens can beimproved in VACV with B8R deficiency.Deletion of dominant epitopes may provide a vector with more efficiency for vaccines and gene therapy.