Expression of RGC32 gene in pulmonary adenocarcinoma tissue and the influence on proliferation and apoptosis of A549 cells
10.3760cma.j.issn.1006-9801.2012.06.002
- VernacularTitle:肺腺癌组织中RGC32基因的表达及其对肺腺癌A549细胞增殖和凋亡的影响
- Author:
Ran XU
;
Jungang ZHAO
;
Wenjun SHI
- Publication Type:Journal Article
- Keywords:
Lung neoplasms;
Adenocarcinoma;
Cell proliferation;
Apoptosis;
Gene,RGC32
- From:
Cancer Research and Clinic
2012;24(6):366-368
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of RGC32 gene in pulmonary adenocarcinoma tissue and to explore the influence on proliferation and apoptosis of A549 cells.Methods Real-time PCR was applied to detect the expression of RGC32 gene in 36 cases of pulmonary adenocarcinoma and pericancerous tissues.RNA interference was used to inhibit the expression of RGC32 gene.After RNA interference,the expression of RGC32 gene was detected by real-time PCR,the apoptosis of the transfected cells was detected by flow cytometry and the inhibition ratio of cell proliferation was detected by methyl thiazolyl tetrazolium (MTT).Results The expression of RGC32 gene was upgraded in pulmonary adenocarcinomas tissues(1:2.2736,t=-29.185,P=0.01).After RNA interference,the expression of RGC32 gene transfected A549 cells was down-regulated significantly[(2.47±0.17)％ vs(4.65±0.26)％,t=-202.868,P=0.000].Comparing to the control cells,the apoptosis of experimental group cells increased significantly (2.9 ％ vs 45.4 ％,t=-37.915,P=0.01),and the inhibition ratio of cell proliferation increased significantly.Conclusion The expression of RGC32 gene shows an obvious upgraded in pulmonary adenocarcinoma.The low expression of RGC32 gene can induce apoptosis and inhibit proliferation of A549 cells.
