The expression of γ-glutamylcysteine synthetase in the skeletal muscles of mice with emphysema and its significance
10.3760/cma.j.issn.0254-1424.2012.07.005
- VernacularTitle:肺气肿小鼠骨骼肌γ谷氨酰半胱氨酸合成酶的表达及意义
- Author:
Jinfeng WANG
;
Yunqiao LI
- Publication Type:Journal Article
- Keywords:
Emphysema;
Chronic obstructive pulmonary disease;
γ-Glutamylcysteine synthetase;
Skeletal muscle
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2012;34(7):498-500
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study any change in the expression of γ-glutamylcysteine synthetase (γ-GCS) in the skeletal muscles of mice with emphysema.MethodsTwenty Kunming male mice were divided into a normal control group and an emphysema group (n =10 in each ).An emphysema model was established by passive cigarette smoking in 8 of the emphysema group mice.TUNEL staining was used to detect apoptotic skeletal muscle cells.Im munohistochemical assays were used to determine the protein level of γ-GCS synthetase.ResultsThe average optical density of apoptotic cells was significantly higher in the skeletal muscles of the mice with emphysema compared with the normal controls,and their average γ-GCS synthetase levels were also significantly higher.Conclusions Apoptotic cells increase in skeletal muscle during emphysema,which may be caused by an oxidation/antioxidant imbalance mediated by γ-GCS synthetase.
