Mimic hypoxia of deferasirox in improving growth of micrangium in a narrow pedicle flap
10.3760/cma.j.issn.1671-0290.2012.04.013
- VernacularTitle:地拉罗司对大鼠窄蒂缺氧皮瓣模型促进微血管生成的实验
- Author:
Zihan XU
;
Tianlan ZHAO
;
Daojiang YU
;
Xiaoming XIE
;
Yu JI
- Publication Type:Journal Article
- Keywords:
Narrow pedicle flap;
Deferasirox;
Hypoxia inducible factor-1a (HIF-1α);
Vascular endothelial growth factor (VEGF);
Microvessel density (MVD)
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2012;18(4):282-285
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the function of hypoxia mimics of different exposure time of deferasirox in improving the growth of micrangium in a narrow pedicle flap.Methods Fourty male SD rats were divided into 2 groups:experimental group was fed with deferasirox 100 mg/kg per day from 1d,3d,5d and 7d,respectively,before the surgery of transferring the narrow pedicle flap,while control group just fed with saline.After 7 days,the immunohistochemistry,Western blot and quantitative reverse transcription-PCR (qPCR) were performed to examine the expression of CD34.qPCR was used to examine the expression of HIF-1α,VEGF in order to investigate the regulatory effect of deferasirox in improving the growth of micrangium and the distinction among the different exposed time of deferasirox in the narrow pedicle flap.Results The deferasirox group exhibited a marked improvement in flap healing time,and with the increasing administration time of deferasirox,the expression of MVD,HIF-1α and VEGF was improved in each treated group (P＜0.05).Conclusions Deferasirox can induce HIF-1α secretion and increase CD34 expression,and so deferasirox can protect endothelial cells from hypoxic and ischaemic injury.
