Effect of Tanreqing on expression of aquaporin-1 in rats with radiation-induced lung injury
10.3760cma.j.issn.1006-9801.2012.06.003
- VernacularTitle:痰热清注射液对早期急性放射性肺损伤大鼠肺组织水通道蛋白1表达的影响
- Author:
Dawei LIU
;
Wen ZHONG
;
Chengying SUN
;
Xiaolan LIU
- Publication Type:Journal Article
- Keywords:
Radiation-induced;
Radiotion-induced lung injury;
Aquaporin 1
- From:
Cancer Research and Clinic
2012;24(6):369-372
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expression of aquaporin-1(AQP1)in rats with acute lung injury induced by radiation,and discuss the protection of Tanreqing for rats with acute lung injury.Methods A total of 20 SD healthy rats were randomly assigned into 4 groups,including the comparison group,the radiation group(0.9％ NaCl 0.2ml),the dexamethasne group(5mg/kg)and the Tanreqing group(0.67ml/kg).Strat intraperitoneal injection one day after radiotherapy,the rats were sacrificed after 7 days.The pathological changes of lung tissues were observed by HE stain.AQP1 expressions in lung tissues were detected by immunohistochemistry and RT-PCR test.Results Compared with the radiation group,there was an obvious amelioration in pathological injury of lung tissue in the dexamethasne and Tanreqing groups.AQP1 was increased significantly in comparison group(0.8006±0.05846),but AQP1 was decreased in the radiation group (0.4938±0.03477)(P＜0.05),and AQP1 was increased after the Tanreqing and dexamethasne treatment(0.6492±0.03477,0.6664±0.03251) (P＜0.05).Conclusion Tanreqing may have beneficial effects on radiationinduced lung injury by regulating AQP1 expression in the lungs of rats.
