Comparation of immunohistochemistry and fluorescence in situ hybridization for detection of Her-2 expression in gastric cancer
10.3760cma.j.issn.1006-9801.2012.06.013
- VernacularTitle:免疫组织化学方法和荧光原位杂交技术检测胃癌组织中Her-2表达的比较
- Author:
Yanfeng XI
;
Wenqi BAI
;
Jing LI
;
Yunfeng BO
;
Yuanyuan ZHAO
;
Wei BAI
- Publication Type:Journal Article
- Keywords:
Stomach neoplasms;
Genes,erbB-2;
In situ hybridization,fluorescence;
Immunohistochemistry
- From:
Cancer Research and Clinic
2012;24(6):402-404
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the association of Her-2 protein expression and gene expression in gastric cancer by different methods,and to explore the effective approaches of Her-2 in gastric cancer detection.Methods Immunohistochemistry (IHC) was used to mark Her-2 protein in 68 cases of gastric cancer,and fluorescence in situ hybridization(FISH)wasused to detect the Her-2 gene amplification.Results Among 68 cases,IHC Her-2 protein in 12 cases(17.65 ％)was positive expression,including ++ 3 cases ++ 9 cases,+ 7 cases and-49 cases.By FISH,Her-2 gene in 11 cases(16.17 ％)was overexpression.Corresponding to the IHC results,FISH results was 3 cases(100 ％),8 cases(88.89 ％),0 cases,0 cases,respectively.Conclusion Her-2 protein expression and gene overexpression occur in gastric cancer.Her-2 protein expression in gastric cancer shows significant heterogeneity.Her-2 gene overexpression and Her-2 protein expression is correlated (P＜0.05).The differences of Her-2 expression in gastric cancer by IHC and FISH mostly occur in IHC++ groupe.Suggestion of Her-2 gene test should be given for patients with IHC++ who will be undertaken Her-2 gene therapy.
