Influence of Grb2-SH3 inhibitor peptidimer-c on the apoptosis-related genes expression in K562 cells
10.3760cma.j.issn.1006-9801.2012.06.001
- VernacularTitle:Grb2-SH3抑制剂peptidimer-c对K562细胞凋亡相关基因表达的影响
- Author:
Shuping CHEN
;
Huijing CHEN
;
Fang LIU
;
Yunbin YE
- Publication Type:Journal Article
- Keywords:
Grb2 adaptor protein;
src homdogy domain;
K562 cells;
Oligonucleotide array sequence analysis;
Gene expression
- From:
Cancer Research and Clinic
2012;24(6):361-365
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the influence of peptidimer-c on the gene expression profiling of K562 cells and investigate the mechanism of peptidimer-c inducing the apoptosis and inhibiting proliferation of K562cells.Methods Trypan blue staining technique was performed for counting the number of living K562 cells treated with peptidimer-c.The ultrastructure changes of K562 cells treated with peptidimer-c was observed under transmission electron microscope.The Human U133 Plus 3.0 gene chips were used to detect the differentially expressed genes of K562 cells treated with peptidimer-c.Reverse transcription PCR was conducted to confirm some genes identified by gene chips.Results Peptidimer-c could induce the apoptosis and inhibit the proliferation of K562 cells.Peptidimer-c caused widely changes of the gene expression profiles of K562 cells.The chip data suggested that there were 529 differentially expressed genes,of which 455 genes were up-regulated and 74 genes were down-regulated.The relevant apoptotic genes were down-regulated markedly,including JUN,AXUD1,TNFRSF10B,etc.Fifteen of the differentially expressed genes were detected by RT-PCR,which was consistent with the chip data.Conclusion Peptidimer-c may induce aooptosis of K562 cells by activating the TNF/TNFR family and the JUN family.
