Expression of serum microRNA-192 in the diabetic nephropathy patients and its impact on TGF-β signaling pathway
10.3760/cma.j.issn.1001-7097.2012.05.008
- VernacularTitle:糖尿病肾病患者血清miR-192的表达及其对转化生长因子β信号通路的影响
- Author:
Jie LI
;
Ping ZHOU
;
Xiumei HU
;
Youming PENG
- Publication Type:Journal Article
- Keywords:
Diabetic nephropathy;
MicroRNAs;
Transforming growth factor-β;
miR-192
- From:
Chinese Journal of Nephrology
2012;28(5):388-391
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the expression of serum microRNA (miR)-192 in diabetic nephropathy (DN) patients and investigate its effects on DN. Methods The serum levels of miR-192 and miR-210 in DN patients,diabetic patients without renal injury and healthy people were detected by real-time quantitative PCR.Down-stream target genes were predicted using TargetScan software and further confirmed by gain of function and loss of function studies in vitro.Mesangial cells were treated with different concentrations of TGF-β1,then miR-192 expressions of cells and supernatant were examined as well. Results Serum miR-192 level significantly decreased in DN patients compared with healthy people (0.41 ±0.09 vs 1.00±0.00,P＜0.01) and diabetic patients without renal injury,while the serum miR-210 levels were not significantly different among three groups.Bioinformatics analysis results showed that some target genes were involved in TGF-β signal pathway.ZBP1,IGF-1 and type Ⅰ collagen (Col Ⅰ ) were chosen and confirmed by Western blotting,which were regulated by miR-192.TGF-β1 decreased the expression of miR-192 in both cells and cell culture supematants. Conclusion Serum miR-192 may promote the progress of DN by regulating TGF-β1 signaling pathway and may be used as a potential biomarker in the diagnosis of DN depending on its certain specificity.
