New model of biliary perfusion systemin vitro
10.3760/cma.j.issn.1007-5232.2012.06.014
- VernacularTitle:一种新型体外胆管灌注系统模型建立的可行性研究
- Author:
Weixing ZHANG
;
Bin LI
;
Baiwen LI
;
Lei LI
;
Xinjian WAN
- Publication Type:Journal Article
- Keywords:
Bile ducts;
In vitro;
Perfusion system;
Experimental model
- From:
Chinese Journal of Digestive Endoscopy
2012;29(6):341-344
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo establish a new model of biliary peffusion in vitro,and to explore the feasibility of comparative biliary studies with the model.MethodsA set of ten-text-area model of biliary peffusion was designed in vitro.Saline,glycerol and pig bile were put into this model.The outflow of the first tube was set at 8 drops/min,and these liquids were collected through the ten sample test areas and measured at 0.5 hour,1.0 hour,1.5 hours,2.0 hours,12.0 hours and 24.0 hours.Equality of liquid amount was evaluated from each sample test area.Rods containing delayed release EDTA of ten different levels were placed in the test areas.The liquid was collected and EDTA concentrations were evaluated from the ten tubes at 24.0 hours.EDTA concentrations of the same rod in the three liquids were compared.ResultsA(1) the same time point,the amounts of saline,glycerol,pig bile flowing through the ten sample test areas showed no significant difference (P ＞ 0.01 ) ; but EDTA concentrations of the same liquid at 24.0 hours were significantly different (P ＜ 0.01 ) ; and EDTA concentrations of the same rod in the three liquids were also obviously different ( P ＜ 0.01 ).ConclusionThe same liquid flow rates and the same experimental environment can be achieved.Different test results can be obtained from different test areas in different experiments.The test results of the same kind of sample change with the changes of experimental conditions,which simulates biliary duct.
