HLA-G 14 bp gene tolvmorthism in relation to Epstein-Barr virus infection in children
10.3760/cma.j.issn.0254-5101.2012.04.017
- VernacularTitle:HLA-G14bp基因多态性与儿童Epstein-Barr病毒感染的相关性分析
- Author:
Huiyan WANG
;
Kegang TIAN
;
Miao FU
;
Yiping CHEN
;
Xiaoqun ZHENG
;
Shijun HE
- Publication Type:Journal Article
- Keywords:
HLA-G;
Gene polymorphism;
Plasma soluble HLA-G;
Epstein-Barr virus;
Infectious mononucleosis
- From:
Chinese Journal of Microbiology and Immunology
2012;32(4):358-362
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship between the HLA-G 14 bp insertion/deletion polymorphism and the infection of Epstein-Barr virus(EBV) for children.Methods The study genotyped HLA-G 14 bp insertion/deletion polymorphism of 102 infectious mononucleosis children and 165 normal controls by PCR-PAGE,detected the plasma sHLA-G level of 51 infectious mononucleosis children and 146 normal controls by ELISA.Results A significant difference was observed for the frequencies of the HLA-G 14 bp genotype between the two groups( x2 =6.742,P=0.034 ),and a significant difference was also observed for the 14 bp allele frequencies between the two groups( x2 =6.672,P=0.01 ).The plasma sHLA-G levels in the infectious mononucleosis children were dramatically higher than that in normal controls,and a significant difference was observed between the two groups( Z=-9.472,P＜0.01 ).Among the infectious mononucleosis children,levels of sHLA-G was find a significant difference between the three genotypes of HLA-G 14 bp insertion/deletion polymorphism( H=6.09,P =0.048 ),and the level of s HLA-G with 14 bp+/+ genotype was markedly lower than that of the two other genotypes (Z=-2.376,P=0.01 8).Conclusion There was a relationship between the HLA-G 14 bp insertion/deletion polymorphism and the susceptibility to the infectious mononucleosis for children.Children who carried the 14 bp-/- genotype or deleted the 14 bp allele may have a significantly increased risk of the infection of EBV.The plasma sHLA-G might be considered as an index for auxiliary diagnosis infectious mononucleosis.
