Expression of stem cell factor and infiltration of mast cells in dermal tissue of rats with chronic renal failure

VernacularTitle:干细胞因子和肥大细胞在慢性肾衰竭大鼠皮肤中的表达和浸润
Author: Yaoxian LIANG ; Xuemei LIU ; Fangxin QIU
Publication Type:Journal Article
Keywords: Kidney failure,chronic; Stem cell factor; Mast cells; Dermal lesions
From: Chinese Journal of Nephrology 2012;28(4):301-304
CountryChina
Language:Chinese
Abstract: Objective To investigate the role of stem cell factor (SCF) and mast cells (MC) in the pathogenesis and progression of dermal lesions caused by chronic renal failure.Methods Thirty-six Wistar rats were randomly divided into model group (adenine lavage at a dose of 150 mg·kg-1·d-1) and control group (physiological saline lavage at equal volume).Six rats from each group were sacrificed respectively at week 4,8 and 12.The intensity of MC infiltration was examined by toluidine blue staining. The expression of SCF was detected by immunohistochemistry and real-time fluorescence quantitative PCR. Results Compared with control group,the intensity of MC and the expression of SCF were significantly higher in dermal tissue of model group (P＜0.O1,respectively),and they were increased with time.In the model group,the number of MC infiltration was positively correlated with both the protein expression of SCF (r=0.81,P＜O.01) and the level of SCF mRNA (r=0.65,P＜0.01). Conclusion The increased SCF and MC may participate in the pathogenesis and progression of dermal lesions caused by chronic renal failure.