The effects of swimming on the expression of glial fibrillary acidic protein and basic fibroblast growth factor after cerebral ischemia and reperfusion
10.3760/cma.j.issn.0254-1424.2012.05.004
- VernacularTitle:不同游泳训练强度对脑缺血再灌注大鼠胶质纤维酸性蛋白及碱性成纤维细胞生长因子表达的影响
- Author:
Peipei WANG
;
Yiling WU
;
Qiang WANG
- Publication Type:Journal Article
- Keywords:
Cerebral ischemia;
Reperfusion;
Training;
Glial fibrillary acidic protein;
Basic fibroblast growth factor;
Swimming
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2012;34(5):334-339
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of different swimming training intensities on the expression of glial fibrillary acidic protein (GFAP) and basic fibroblast growth factor (bFGF) around a cerebral infarct. Methods The intraluminal thread method was applied to establish left middle cerebral artery occlusion (MCAO) for 2 h in 180 rats.They were then reperfused for 3,7 and 14 days.The rats were divided into four groups ( Ⅰ -Ⅳ) according to the intensity of the swimming training they were required to do,plus a control group and a sham operation group.The rats in training group Ⅰ swam 5 min,once daily; group Ⅱ trained for 5 min twice daily; group Ⅲ swam for 10 min once daily and group Ⅳ trained for 10 min twice daily.Neurological function was evaluated using Bederson's test,the cerebral infarction volume was calculated by tripeny tetrazolium chloride (TTC) staining.The expression of GFAP and bFGF around the infract were detected using immunohistochemistry.ResultsThe average Bederson scores of the exercise training groups on the 7th and 14th days were significantly lower than that of the control group.The infarction volumes of each exercise training group were significantly lower than that of the control group,and cells positive for GFAP and bFGF in all training groups were significantly more numerous on the 3th,7th and 14th day after MCAO,especially in training group Ⅳ.Conclusion Intensive exercise can adjust the expression of GFAP and bFGF and promote the repair of brain damage after cerebral ischemia and reperfusion.
