The regulation of Kir4.1 by pigment epithelium-derived factor in Müller cells under high glucose conditions
10.3760/cma.j.issn.1005-1015.2012.03.014
- VernacularTitle:色素上皮衍生因子对高糖环境下视网膜Müller细胞钾离子通道Kir4.1的调控
- Author:
Xi SHEN
;
Yana WANG
- Publication Type:Journal Article
- Keywords:
Diabetic retinopathy/pathophysiology;
Cytokines/physiology;
Müller cells;
Potassium channels/physiology
- From:
Chinese Journal of Ocular Fundus Diseases
2012;28(3):268-271
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate Kir4.1 expressions in Müller cells under high glucose conditions and treatment of pigment epithelium derived factor (PEDF).Methods Cultured rat Müller cells were divided into control group (5 mmol/L glucose),high glucose group (25 mmol/L glucose),PEDF treatment group (25 mmol/L glucose+ 100 ng/ml PEDF) and intervention control group(25 mmol/L glucose +phosphate buffer solution). Kir4.1 expressions were measured by Western blot and real-time reverse transcription polymerase chain reaction (RT-PCR). Reactive oxygen species (ROS) productions were measured using 2′7′-dichlorofluorescin diacetate and glutathione peroxidase (GPx)expressions were studied by real-time RT-PCR.ResultsBy Western blot and real-time RT-PCR,it was found the expressions of Kir4.1 decreased obviously under high glucose conditions (real-time RT-PCR:t=4.12,P＜0.05; Western blot:t=3.53,P＜0.05) ; simultaneously,ROS generation was increased (t=3.76,P＜0.05) and GPx level was decreased (t=3.18,P＜0.05).PEDF treatment inhibited the high glucose-induced Kir4.1 down regulation (real-time RT-PCR:t =3.66,P＜0.05 ; Western blot:t =6.43,P＜0,01 ) and decreased ROS generations (t=4.11,P＜0.05) and increased GPx levels (t=5.12,P＜0.01).ConclusionsThe high glucose can supress Kir4.1 expressions in Müller cells by oxidative stress,and PEDF can ameliorate these effects.
