Effect of selective cycloxygenase-2 inhibitor NS-398 on IL-10 and IFN-γ mRNA expression in hunman keratinocytes induced by long-wave ultraviolet
10.3760/cma.j.issn.1671-0290.2012.03.014
- VernacularTitle:NS398对长波紫外线诱导的HaCaT细胞白介素-10及γ干扰素mRNA表达的影响
- Author:
Shanshan WANG
;
Hong LIANG
;
Xing DAI
;
Xiaoming LIU
;
Yinhua SHONG
- Publication Type:Journal Article
- Keywords:
Long wave ultraviolet;
NS398;
Interleukin-10;
Interferon type Ⅱ
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2012;18(3):204-207
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of selective COX-2 inhibitor NS398 on IL-10 and IFN-γexpression in human keratinocytes induced by UVA,and further to explore the effect on anti human skin photoaging.Methods HaCaT cells were divided into three groups:simple UV exposure group,NS398-intervene group and blank group.For UV radiation,simple UV exposure group and NS398-intervene group were irradiated with UVA at a dose of 30 J/cm2.In order to assess the effect of NS398,HaCat cells were treated with NS398 at the dose of 0,20,40,80μmol/L respectively and then incubated without irradiation for 2h,substrate changed and cultured for 24h,and then the expression of IL-10 and IFN-γ mRNA was detected via RT-PCR.Results Simple UV exposure group had obviously higher expression of IL-10 mRNA and lower expression of IFN-γ mRNA than that of control group,while NS398-intervened group had significantly lower expression of IL-10 mRNA and higher expression of IFN-γ mRNA than that of simple UV exposure group,and dose-dependency existed.Conclusions NS398 may delay photo-damage of human skin via inhibiting the expression of IL-10 mRNA and up-regulating the expression of IFN-γ mRNA in human keractinocytes.
