A methodology for dynamic evaluation of the biological efficacy of botulinum toxin and its dose-effect relationship
10.3760/cma.j.issn.0254-1424.2012.05.001
- VernacularTitle:肉毒毒素生物效应的动态观测模型的构建及其量效关系
- Author:
Lingjing JIN
;
Lei ZHANG
;
Wuchao LIU
;
Qiang GUAN
;
Lizhen PAN
;
Qing ZHAN
;
Zhiyu NIE
- Publication Type:Journal Article
- Keywords:
Botulinum toxin A;
Biological efficacy;
Dose-response relationships
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2012;34(5):321-324
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a method for dynamically observing the biological efficacy of botulinum toxin A (BTX-A) and to investigate the dose-effect relationship between BTX-A dosage and muscle strength.MethodsFifty-four male Sprague Dawley rats were randomly divided into 9 groups.Groups 1-7 were injected intramuscularly with 0.1 ml BTX-A (0.01 U to 4.0 U) into the gastrocnemius on the right side.Rats in group 8 were injected intramuscularly with an equal volume of saline solution as the control group,and group 9 was used to determine the location of injection.Gastrocnemius muscle strength was evaluated using a self-made evaluation system before and after the toxin injection and on the 3rd,7th,14th,21st,30th,45th,60th and 75th day following.ResultsMuscle strength reached its lowest level on days 3 to 7,with a significant difference in the decline of muscle strength between the test groups and the control group up to day 60.With the lower BTX-A doses (0.01 U,0.1 U,0.5 U,1.0 U),muscle strength had decreased significantly on the 21st day,but recovered to its initial levels in all groups at the same time.There was no significant difference among the 1.0 U,1.5 U,2.0 U and 4.0 U groups.ConclusionsStandardized gastrocnemius injection combined with neuromuscular functional evaluation can establish a model of BTX-A dosage and muscle paralysis which can be used to assess the evolution of the biological efficacy of BTX-A.
