The effects of osteogenic growth peptide combined with extracorporeal shock waves on osteoblast proliferation
10.3760/cma.j.issn.0254-1424.2012.05.005
- VernacularTitle:成骨生长肽联合体外冲击波治疗对成骨细胞增殖功能的影响
- Author:
Jun ZHONG
;
Shiqing LIU
;
Jinliang WEI
;
Ruicheng YAN
- Publication Type:Journal Article
- Keywords:
Osteogenic growth peptide;
Extroacorporeal shock waves;
Protein kinase A;
MC3T3-E1cells
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2012;34(5):340-343
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of osteogenic growth peptide (OGP) in combination with extracorporeal shock waves (ESWs) on osteoblast proliferation.Methods Passaged cells were divided into four groups for different treatments:a control group,an OGP + ESW group,an ESW group,and an OGP group.After the respective treatments,the cells were cultured for 24 h,48 h and 72 h and counted using methylthiazdy tetrazolium (MTT) and an inverted fluorescence microscope. Immunohistochemical examination was used for detecting protein kinase A (PKA)activity,and a reverse transcription-polymerase chair reaction (RT-PCR) was used for examining PKA mRNA expression at 24 and 48 hours.Results Cell counting revealed that cell proliferation in the OGP + ESW,ESW and OGP groups was significantly promoted compared with the control group.Cell proliferation was greatest in the OGP + ESW group.The immunohistochemical examination showed positive staining intensities in the OGP + ESW,ESW and OGP groups significantly higher than in the control group.The positive staining intensity in the OGP + ESW group was again the highest.PKA activity was also significantly higher in the OGP + ESW,ESW and OGP groups than in the control group with the level in the OGP + ESW group the highest.Conclusion OGP in combination with ESW has a synergistic effect in stimulating osteoblast proliferation and growth.
