Υδ T cells play an important role in the development of RSV-influenced allergic airway inflammation
10.3760/cma.j.issn.0254-5101.2012.03.011
- VernacularTitle:γδ T细胞参与呼吸道合胞病毒感染对气道变态炎症反应的影响作用
- Author:
Leiying ZHANG
;
Jianqi WU
;
Jing LIU
;
Bing WANG
;
Sheng ZENG
;
Beixing LIU
- Publication Type:Journal Article
- Keywords:
Asthma;
γδ T cell;
Respiratory syncytial virus;
Inflammation
- From:
Chinese Journal of Microbiology and Immunology
2012;32(3):234-238
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate whether γδ T cells act as a regulatory factor during respiratory syncytial virus (RSV) infections was responsible for the subsequent changes in asthmatic-type inflammation in allergic mice.MethodsMice were sensitized and challenged with OVA,and infected intranasaly with RSV before or after OVA sensitization.Lung sections were stained with HE for determination of inflammatory reaction.Real-time RT-PCR was used to analyze the expression of cytokine mRNA of γδ T cells in the lung and spleen of tested mice.The number of γδ T cells in the spleen and lung of BALB/c mice was determined by flow cytometry.Adoptive transfer of γδ T cells was performed to identify the role of γδ T cells in allergic asthma.ResultsOVA-sensitized and challenged mice exhibited significantly peribronchial inflammation with larger number of mononuclear cells and granulocytes in the lung tissue sections.RSV infection before OVA-sensitization diminished the grade of inflammatory responses induced by OVA treatment.The expression of IFN-γ mRNA was increased siguificantly in RSV-infected,OVA-sensitized mice.In contrast,the level of IL-4 mRNA was diminished.The number of total γδ T cells as well as activated γδ T cells was decreased in the spleen and lung of OVA-sensitized mice by prior RSV infection.Adoptive transfer of γδ T cells obtained from OVA-sensitized and challenged mice induced a slight inflammation in the lung of normal mice,and enhanced inflammatory responses in RSV-infected OVA-sensitized mice.Conclusionγδ T cells may play an important role in the development of allergen-induced allergic airway inflammation.
