Hypoxia can induce EMT and promote invasion of breast cancer cells

VernacularTitle:缺氧对乳腺癌细胞侵袭和迁移能力影响的研究
Author: Fuqiang JIANG ; Juyi WEN ; Jingbo KANG ; Qing NIE
Publication Type:Journal Article
Keywords: Breast neoplasms; Anoxia; Epithelium; Stromalcells; Neoplasm invasiveness; Cel migration
From: Cancer Research and Clinic 2012;24(4):217-219
CountryChina
Language:Chinese
Abstract: Objective To study the effect of hypoxia on EMT molecule E-cadherin, Vimentin and invasion of human breast cancer MCF-7 cells, reveal the mechanism of breast cancer invasion and metastasis and provide experimental and theoretical basis. Methods Western blot was used to observe the change of HIF-1α, E-cadherin and Vimeutin during hypoxia on MCF-7. MTT was used to study the effects of viability.Transwell chamber was used to detect the ability of invasion and metastasis. Results The expression of E-cadherin was significantly lower (0.09±0.02)(t=30.98,P=0.0007) and the expression of Vimentin was significantly higher (0.69±0.04) (t=915,P=0.0000) with the extension of hypoxia.The capability of adhesion (81.23±0.74) (t=82.05,P=0.000),invasion(120±6) (t=22.78,P=0.0009) and migration(190±6) (t=23.49,P=0.000)was significantly increased after 72 h hypoxia(P＜0.05).Conclusion Hypoxia can downregulate E-cadherin and upregulate Vimentin and enhance the adhesion,invasion and migration of MCF-7.