Detection of circulating tumor cells from peripheral blood in patients with esophageal squamous cell carcinoma and its clinical significance
10.3760/cma.j.issn.1001-4497.2012.03.009
- VernacularTitle:食管鳞癌外周血循环肿瘤细胞检测及临床意义
- Author:
Jun LI
;
Wei WANG
;
Yuanyuan QIAO
;
Dajin ZHANG
;
Feng WEN
;
Liqun SHANG
;
Xuechang LI
;
Junqiang LIU
- Publication Type:Journal Article
- Keywords:
Circulating tumor cells(CTCs);
Esophageal squamous cell carcinoma(ESCC);
Negative selection
- From:
Chinese Journal of Thoracic and Cardiovascular Surgery
2012;28(3):157-160
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the relationship between circulating tumor cells(CTCs) in the peripheral blood of patients with esophageal squamous cell carcinoma(ESCC) and the physiopathological characteristics of esophageal neoplasms.MethodsUsing negative selection system,we depleted red blood cells(RBCs) in red blood cell lysis buffer,depleted white blood cells (WBCs) with Miltenyi magnetic beads and enriched the rare cells from ESCC patients'peripheral blood.Immunofluorence staining (IF) was adopted to identify CTCs.ResultsCirculating tumor cells in the peripheral blood of patients with esophageal squamous cell carcinoma was closely related to cell differentiation grade,the invasion of primary cancer,lymph node status,P-TNM stages,and was rarely related to the sex,age or the location of tumor.ConclusionThe results suggest that circulating tumor cells in the peripheral blood of patients with esophageal squamous cell carcinoma may express the development of esophageal cancer and may be served as a tumor marker to evaluate the biological behavior of esophageal cancer.
