DNA damage in peripheral blood lymphocytes of patients with autosomal dominant polycystic kidney disease
10.3760/cma.j.issn.1001-7097.2012.03.002
- VernacularTitle:常染色体显性遗传多囊肾病患者外周血淋巴细胞DNA的损伤
- Author:
Ming LI
;
Songbing QIN
;
Lili WANG
;
Guoyuan LU
;
Qing QIAO
;
Lei SHEN
- Publication Type:Journal Article
- Keywords:
Polycystic kidney,autosomal dominant;
Lymphocytes;
DNA damage;
Single-cell gel electrophoresis
- From:
Chinese Journal of Nephrology
2012;28(3):170-173
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect DNA damage of peripheral blood lymphocytes in patients and family members of autosomal dominant polycystic kidney disease (ADPKD),and to study the effect of irradiation on genomic stability of lymphocytes. Methods Before and after 0.5 Gy radiation dose,single-cell gel electrophoresis (SCGE) was employed to analyze DNA damage of lymphocytes in 10 ADPKD patients (group A),3 members without clinical symptoms of a ADPKD family (group B) and 20 healthy control people (group C).The damage was estimated based on the content of DNA in tail (TDNA％) with comet analysis software (CASP). Results Both before and after irradiation,the TDNA％ (8.85％±0.14％,14.84％±0.77％) and the value-added (6.00％±0.77％) of TDNA％ of group A were significantly higher than those of group C (7.50％±0.37％,12.46％±0.26％,4.96％±0.44％) respectively.There were no significant differences between group B and group C or group A and group B.While 1 person in group B had higher TDNA％ as compared to group C both before and after irradiation. Conclusions The lymphocytes of ADPKD patients are more sensitive to ionizing radiation as compared to healthy people.The genomic instability in ADPKD patients or member of ADPKD family may trigger cystic formation in multi-organs when exposing to environmental agents. SCGE may provide a new approach to elucidate the pathogenesis and prognosis of ADPKD.
