Effect of pigment epithelium-derived factor on p38MAPK-CREB pathway and fibronectin in high glucose cultured human mesangial cells
10.3760/cma.j.issn.1001-7097.2012.03.010
- VernacularTitle:色素上皮衍生因子对高糖诱导人肾小球系膜细胞p38丝裂原活化蛋白激酶-cAMP反应元件结合蛋白通路及纤连蛋白的影响
- Author:
Lan GAO
;
Jing LI
;
Ling GAO
;
Hongmin CHEN
;
Lian HONG
- Publication Type:Journal Article
- Keywords:
Glomerular mesangial cells;
p38 mitogen-activated protein kinases;
Cyclic AMP response element-binding protein;
Fibronectins;
Pigment epithelium- derived factor
- From:
Chinese Journal of Nephrology
2012;28(3):212-216
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of pigment epithelium- derived factor (PEDF) on p38MAPK-CREB pathway and the expression of fibronectin (FN) in human mesangial cells (HMCs) cultured with high glucose. Methods HMCs were treated with different concentrations of glucose and the osmotic control respectively in the presence or absence of PEDF for 24 h:normal glucose (5.6 mmol/L),24.4 mmol/L mannitol,high glucose (30 mmol/L),high glucose+PEDF(30 mmol/L glucose with 10 nmol/L PEDF,40 nmol/L PEDF or 100 nmol/L PEDF).After samples were collected,the expression of phospho-p38MAPK (p-p38) and p-CREB was assessed by Western blotting,while FN mRNA and protein expression was assessed with RT-PCR and ELISA respectively. Results In contrast to normal glucose and mannitol treatments,the expression of p-p38MAPK,p-CREB and FN increased significantly in high glucose group (all P＜ 0.01).However,PEDF abolished the up-regulation of p-p38MAPK,p-CREB and FN induced by high glucose (all P＜0.05). Conclusion PEDF may inhibit fibrosis through P38MAPK-CREB pathway in diabetic nephropathy.
