Establishment and evaluation of implanted inferior vena cava tumor thrombus model in New Zealand white rabbits
10.3760/cma.j.issn.1006-9801.2012.02.003
- VernacularTitle:新西兰大白兔植入性下腔静脉癌栓模型的建立及评估
- Author:
Wen ZHANG
;
Zhiping YAN
;
Jianjun LUO
;
Zhuting FANG
;
Linlin WU
;
Qingxin LIU
;
Xudong QU
;
Jianhua WANG
- Publication Type:Journal Article
- Keywords:
Cell line,VX2;
Models,animal;
Vena cava,inferior;
Neoplastic cells,circulating;
Tomography,spiral computed
- From:
Cancer Research and Clinic
2012;24(2):80-83
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo establish an animal model of implanted inferior vena cava tumor thrombus (IVCTT)and examine its growth with MDCT and 3D-MPR. MethodsTumor cell line VX2 was inoculated subcutaneously into rabbit to develop the primary tumor, which was then cut into small strips. Purse-string suture was performed on the anterior wall of IVC after the laparotomy in eighteen New Zealand white rabbits.The tumor strip was injected into IVC through the purse and suspensory fixed on the inner wall of IVC. The general conditions,body weight,and the survival time were monitored after operations.MDCT examinations were performed with plain scan,arterial phase,portal phase and venous phase enhancement every week for all animals and 3D-MPR were acquired.The volumes of IVCTT were calculated.IVC,IVCTT and metastasis were examined with gross and histological pathology. ResultsThe IVCTT was confirmed by MDCT and 3D-MPR images.Collateral varicose veins caused by IVC obstruction and metastasis were also shown in images.IVCTT and metastasis were confirmed by pathological method. The success rate of IVCTT was 100 ％. The mean survival time of operated animals was(49.5±4.4)days. ConclusionsInjecting and suspensory fixing VX2 tumor strip into IVC is a reliable method to establish the IVCTT animal model. MDCT and 3D-MPR are valuable methods to monitor the growth and metastasis of IVCTT in animal models. The model of implanted IVCTT of rabbits provides a useful tool for the research of treatment of IVCTT.
