The role of NF-κB signaling pathway protein and cytokines in Staphylococcus aureus Panton-Valentine leukocidin-induced lung inflammation and injury
10.3760/cma.j.issn.0254-5101.2012.01.010
- VernacularTitle:NF-κB信号通路蛋白和细胞因子在金黄色葡萄球菌PV杀白细胞素相关肺炎症性损伤中的作用
- Author:
Cuiping ZHANG
;
Xiaoling MA
;
Wenjiao CHANG
;
Xin ZHOU
- Publication Type:Journal Article
- Keywords:
Staphylococcus aureus;
Panton-Valentine leukocidin;
Cytokine;
Nuclear transcription factor-κB
- From:
Chinese Journal of Microbiology and Immunology
2012;32(1):43-47
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the role of NF-κB signaling pathway protein and cytokines in Panton-Valentine leukocidin (PVL)-induced acute lung inflammation and injury.MethodsThirty rabbits were distributed randomly into two groups,each group had fifteen rabbits.Group rPVL were directly treated with endotracheal instillation of rPVL,normal control were treated with PBS.Then five rabbits chosen at random from each group were killed at 3,6,or 9 h postinfection.The lung was removed from the rabbits to determine histopathology studies.ELISA was performed to evaluate levels of IL-6,IL-8,IL-10 and TNF-α.NF-κB p65 protein of the lung tissue was assessed by immunohistochemistry method.ResultsIn group rPVL histopathology study showed symptoms of severe illness:diffuse infiltration of inflammatory cells,hemorrhage,edema and other manifestations of lung injury.Levels of IL-6,IL-8 and TNF-α were increased gradually,and the level of IL-10 was increased at 9 h postinfection.The expression of NF-κB p65 protein was increased gradually with the infection time.ConclusionNF-κB activation and cytokines release play an important role in PVL-related lung injury.It may be an important path to down regulate the counts of NF-κB activation.
