The effects of exercise on the activity of heme oxygenases and the expression of heme oxygenase-1 mRNA in vascular smooth muscle tissue of rats with spontaneous hypertension
10.3760/cma.j.issn.0254-1424.2012.02.001
- VernacularTitle:运动对自发性高血压大鼠血管平滑肌血红素氧合酶-1活性及血红素氧合酶-1mRNA表达的影响
- Author:
Cailing REN
;
Weidong TANG
;
Jun ZHANG
- Publication Type:Journal Article
- Keywords:
Heme oxygenase;
Hypertension;
Exercise
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2012;34(2):81-84
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of exercise on the activity of heme oxygenase-1 ( HO-1 ) and the expression of HO-1 mRNA and the mechanisms involved in the vascular smooth muscle tissue of rats with spontaneous hypertension (SHR). Methods Twelve male Wistar rats with normal blood pressure were used as control group ( group C ).Another 24 male rats with SHR were randomly assigned to one of 2 experimental groups ( 12 rats per group):an SHR group (group S) and an SHR treated group (group T).Rats in group T were treated with 60 minutes of unloaded swimming exercise 6 times a week for 9 weeks.Their blood pressure was measured once a week.After the nine weeks HO-1 activity as well as the expression of HO-1 mRNA in vascular smooth muscle and the concentration of carbon monoxide in plasma were measured. Results After the 9 weeks of training,average systolic blood pressure in group T was close to that of group C.The systolic blood pressure of group S continued to rise,and was significantly higher than that of the other 2 groups at each time point.Average HO-1 activity in group S (637.94 ± 73.637 ) reduced significantly compared with that of group C (786.20 ± 74.698) or with that of group T ( 1036.53 ± 140.63 ).That of group T was significantly higher than that of group C.The average expression of HO1 mRNA in group S (80.85 ± 6.953 ) was significantly higher than that of group C (45.15 ± 7.651 ) and lower than that of group T (90.70 ± 11.20),and the differences were statistically significant at the 5％ level of confidence.The average level of expression of HO-1 mRNA in the T group was significantly higher than that of group C.The plasma carbon monoxide content of S group was significantly lower than that of groups C and T. Conclusions Exercise can enhance the activity of HO-1 and the expression of HO-1 mRNA in vascular smooth muscle in rats with SHR to reduce blood pressure.
